B52_puro_empty_gRNA
(Plasmid #197557)

• Purpose: (Empty Backbone) sites for cloning two gRNA with puromycin cassette
• Depositing Lab: Darius Bägli
• Publication: Persistent changes in KCNB2 expression and CpG methylation is associated with ongoing hypertrophic changes after release of partial outlet obstruction. (unpublished)

Backbone

• Vector backbone: B52 with 2 gRNA cloning sites
• Backbone manufacturer: Alberto Ciccia, ADDGENE #100708
• Backbone size (bp): 2633
• Modifications to backbone: addition of puromycin resistance cassette under control of CMV promoter.
• Vector type: Mammalian Expression ; empty gRNA backbone with puromycin resistance gene
• Promoter: U6 for gRNA, CMV for puromycin resistance.
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: searching for
• 3′ sequencing primer: searching for

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This is a derivative of B52 from ADDGENE catalogue# 100708, which then had CMV-puromycin added into it.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Any publications using this clone should state: "we thank the laboratory of Darius Bagli for the use of this clone". In addition, we have a forthcoming article which can be referenced once it is published.