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Myc-Bnip3-T181A
(Plasmid #197556)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197556 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 5964
  • Modifications to backbone
    Myc tag added to multiple cloning region
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BCL2 Interacting Protein 3
  • Alt name
    Bnip3, Nip3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    564
  • Mutation
    Threonine 181 changed to alanine
  • GenBank ID
    NM_009760.4
  • Entrez Gene
    Bnip3 (a.k.a. Nip3)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myc-Bnip3-T181A was a gift from Joseph Gordon (Addgene plasmid # 197556 ; http://n2t.net/addgene:197556 ; RRID:Addgene_197556)

  • For your References section:

    Misoprostol treatment prevents hypoxia-induced cardiac dysfunction through a 14-3-3 and PKA regulatory motif on Bnip3. Martens MD, Seshadri N, Nguyen L, Chapman D, Henson ES, Xiang B, Falk L, Mendoza A, Rattan S, Field JT, Kawalec P, Gibson SB, Keijzer R, Saleem A, Hatch GM, Doucette CA, Karch JM, Dolinsky VW, Dixon IM, West AR, Rampitsch C, Gordon JW. Cell Death Dis. 2021 Nov 26;12(12):1105. doi: 10.1038/s41419-021-04402-3. 10.1038/s41419-021-04402-3 PubMed 34824192
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