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Addgene

human STIM1 CFP
(Plasmid #19755)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19755 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MO91
  • Backbone manufacturer
    See author's map
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STIM1
  • Alt name
    stromal interaction molecule 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
  • Tag / Fusion Protein
    • CFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Author's Map for empty backbone (MO91) information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human STIM1 CFP was a gift from Anjana Rao (Addgene plasmid # 19755 ; http://n2t.net/addgene:19755 ; RRID:Addgene_19755)

  • For your References section:

    Orai1 is an essential pore subunit of the CRAC channel. Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan PG. Nature. 2006 Sep 14. 443(7108):230-3. 10.1038/nature05122 PubMed 16921383
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