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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMO91
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Backbone manufacturerSee author's map
- Backbone size w/o insert (bp) 6000
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSTIM1
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Alt namestromal interaction molecule 1
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SpeciesH. sapiens (human)
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Entrez GeneSTIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See Author's Map for empty backbone (MO91) information.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
human STIM1 YFP was a gift from Anjana Rao (Addgene plasmid # 19754 ; http://n2t.net/addgene:19754 ; RRID:Addgene_19754) -
For your References section:
Orai1 is an essential pore subunit of the CRAC channel. Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan PG. Nature. 2006 Sep 14. 443(7108):230-3. 10.1038/nature05122 PubMed 16921383
Map uploaded by the depositor.
