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Addgene

pEPSW1KN0024
(Plasmid #197539)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCK1
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLP7::LucN::FLAG::nosT
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.02.13.528283 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEPSW1KN0024 was a gift from Nicola Patron (Addgene plasmid # 197539 ; http://n2t.net/addgene:197539 ; RRID:Addgene_197539)

  • For your References section:

    Tuning Plant Promoters Using a Simple Split Luciferase Method to Assess Transcription Factor-DNA Interactions. Cai YM, Witham S, Patron NJ. ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 10.1021/acssynbio.3c00094. Epub 2023 Oct 19. 10.1021/acssynbio.3c00094 PubMed 37856867
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