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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19753 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLB2 FLIP
- Backbone size w/o insert (bp) 10463
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Vector typeMammalian Expression, Mouse Targeting, Lentiviral, RNAi, Cre/Lox
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Growth instructionsgrow in stbl3 cells at 30oC
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameoligo targeting Firefly luciferase
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Insert Size (bp)386
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer GFP miR primer (GCTGGAGTTCGTGACCGCC) (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Lentiviral vector that constitutively expresses puromycin-resistance and surface Thy1.1 driven by the UbiquitinC promoter. Recombines to express GFP and miR30-based RNAi.
See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLB2 Ubi-FLIP FF was a gift from Richard Hynes (Addgene plasmid # 19753 ; http://n2t.net/addgene:19753 ; RRID:Addgene_19753) -
For your References section:
A system for Cre-regulated RNA interference in vivo. Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO. Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. 10.1073/pnas.0806907105 PubMed 18779577