pLB2 CAG P2Gm
(Plasmid #19752)

• Purpose: (Empty Backbone)
• Depositing Lab: Richard Hynes
• Publication: Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.

Backbone

• Vector backbone: LB2
• Backbone size (bp): 10552
• Vector type: Mammalian Expression, Mouse Targeting, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: grow in stbl3 cells at 30oC
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: GFP miR primer (GCTGGAGTTCGTGACCGCC)

Resource Information

• Supplemental Documents:
  • FLIP vector protocols
• Addgene Notes:
  • Addgene Diagnostic Test
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lentiviral vector that constitutively expresses puromycin-resistance and GFP driven by the CAGGS promoter.

See FLIP vector protocols (PDF from this page) for more information.

Firefly hairpin sequence is -
AAGGTATATTGCTGTTGACAGTGAGCGAGCTCCCGTGAATTGGAATCCTA
GTGAAGCCACAGATGTAGGATTCCAATTCAGCGGGAGCCTGCCTACTGCC
TCG