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AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)
(Plasmid #197511)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197511 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Cbh_v5 AAV-ABE C-terminal (Addgene Plasmid #137178)
  • Vector type
    AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
  • Alt name
    CA20
  • Alt name
    C-term AAV-SpRY-gRNAentry
  • Species
    Synthetic
  • Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R)
  • Promoter Cbh
  • Tags / Fusion Proteins
    • BPNLS-NpuC(intein) (N terminal on insert)
    • BPNLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer oBK7321-GGGCTGTAATTAGCTGAGCAAGAGG
  • 3′ sequencing primer oBK219-GGGAGTGGCACCTTCCAGGGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-pCbh-SpRY[C-term]-gRNAentry (CA20) was a gift from Benjamin Kleinstiver (Addgene plasmid # 197511 ; http://n2t.net/addgene:197511 ; RRID:Addgene_197511)

  • For your References section:

    Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy. Alves CRR, Ha LL, Yaworski R, Sutton ER, Lazzarotto CR, Christie KA, Reilly A, Beauvais A, Doll RM, de la Cruz D, Maguire CA, Swoboda KJ, Tsai SQ, Kothary R, Kleinstiver BP. Nat Biomed Eng. 2023 Dec 6. doi: 10.1038/s41551-023-01132-z. 10.1038/s41551-023-01132-z PubMed 38057426
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