AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)
(Plasmid #197509)

• Purpose: Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)
• Depositing Lab: Benjamin Kleinstiver
• Publication: Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038/s41551-023-01132-z.

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Backbone

• Vector backbone: Cbh_v5 AAV-ABE N-terminal (Addgene Plasmid #137177)
• Vector type: AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
• Alt name: HES1468
• Alt name: N-term AAV-ABE8e-nSpCas9(D10A)
• Species: Synthetic
• Mutation: ABE8e mutations in TadA and nSpCas9(D10A)
• Promoter: Cbh
• Tags / Fusion Proteins:
  • BPNLS (N terminal on insert)
  • NpuN(intein)-BPNLS (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: oBK7321-GGGCTGTAATTAGCTGAGCAAGAGG
• 3′ sequencing primer: oBK219-GGGAGTGGCACCTTCCAGGGTC

Resource Information

• Supplemental Documents:
  • AAV-pCBh-ABE8e-nSpCas9(D10A)(N-term) (HES1468).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.01.20.524978v1 for bioRxiv preprint.