MSCV P2Gm FF
(Plasmid #19750)

• Depositing Lab: Richard Hynes
• Publication: Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.

Backbone

• Vector backbone: MSCV P2Gm
• Backbone manufacturer: MSCV2.2 with WPRE cloned into ClaI site is the base vector
• Backbone size w/o insert (bp): 7518
• Vector type: Mammalian Expression, Retroviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: grow in stbl3 cells at 30oC
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: oligo targeting Firefly luciferase
• Insert Size (bp): 100

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GFP miR primer (GCTGGAGTTCGTGACCGCC)

Resource Information

• Supplemental Documents:
  • FLIP vector protocols
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MSCV-based entry vector for cloning and validating miR30-based RNAi constructs. The vector expresses puromycin-resistance and GFP, separated by the FMDV 2A peptide.

This is considered the "empty" vector and can be used for cloning. It contains a hairpin targeting Firefly luciferase.

See FLIP vector protocols (PDF from this page) for more information.

There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.