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pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)
(Plasmid #197499)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197499 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV_ABEmax_P2A_GFP (Addgene Plasmid #112101)
  • Vector type
    Mammalian Expression, CRISPR ; in vitro transcription; T7 promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
  • Alt name
    RMD47
  • Alt name
    ABE8.20m-NRRH
  • Species
    Synthetic
  • Mutation
    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I322V/S409I/E427G/R654L/R753G/R1114G/D1135N/V1139A/D1180G/E1219V/Q1221H/A1320V/R1333K)
  • Promoter CMV and T7
  • Tags / Fusion Proteins
    • BPNLS (N terminal on insert)
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer oBK6928-CCAAGTCTCCACCCCATTGACG
  • 3′ sequencing primer oBK219-GGGAGTGGCACCTTCCAGGGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47) was a gift from Benjamin Kleinstiver (Addgene plasmid # 197499 ; http://n2t.net/addgene:197499 ; RRID:Addgene_197499)

  • For your References section:

    Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy. Alves CRR, Ha LL, Yaworski R, Sutton ER, Lazzarotto CR, Christie KA, Reilly A, Beauvais A, Doll RM, de la Cruz D, Maguire CA, Swoboda KJ, Tsai SQ, Kothary R, Kleinstiver BP. Nat Biomed Eng. 2023 Dec 6. doi: 10.1038/s41551-023-01132-z. 10.1038/s41551-023-01132-z PubMed 38057426
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