H2B-SNAPf-mTurquoise2
(Plasmid
#197494)
-
PurposeExpressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the nucleus.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 197494 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSNAPf
-
SpeciesH. sapiens (human)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.01.31.526423 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
H2B-SNAPf-mTurquoise2 was a gift from Pablo Rivera-Fuentes (Addgene plasmid # 197494 ; http://n2t.net/addgene:197494 ; RRID:Addgene_197494) -
For your References section:
A general strategy to develop fluorogenic polymethine dyes for bioimaging. Martin A, Rivera-Fuentes P. Nat Chem. 2024 Jan;16(1):28-35. doi: 10.1038/s41557-023-01367-y. Epub 2023 Nov 27. 10.1038/s41557-023-01367-y PubMed 38012391