1GFP-His-GFP-TEV-cs-ATG3_Y210A
(Plasmid
#197474)
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PurposeBacterial Expression of human ATG3 Y210A
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbone1GFP
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATG3
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SpeciesH. sapiens (human)
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MutationY210A
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Entrez GeneATG3 (a.k.a. APG3, APG3-LIKE, APG3L, PC3-96, hApg3)
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Tag
/ Fusion Protein
- His-GFP (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
1GFP-His-GFP-TEV-cs-ATG3_Y210A was a gift from James Hurley (Addgene plasmid # 197474 ; http://n2t.net/addgene:197474 ; RRID:Addgene_197474)