Flag- MCL1-3A (S159, S162, T163 mutated to A)
(Plasmid #197458)

• Purpose: MCL-1 phosphodegron mutants
• Depositing Lab: Omar Khan
• Publication: Keyan et al Cancers (Basel). 2023 Jan 13;15(2):505. doi: 10.3390/cancers15020505.

Backbone

• Vector backbone: pCMV-Tag2A
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4300
• Total vector size (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MCL1
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: S159A, S161A, T163A
• GenBank ID: NM_021960
• Entrez Gene: MCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BAMH1 (not destroyed)
• 3′ cloning site: ECOR1 (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original plasmid is from Roger Davies lab, obtained from Addgene. The mutations were done in Dr Khan's lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry