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Flag- MCL1-3A (S159, S162, T163 mutated to A)
(Plasmid #197458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag2A
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Total vector size (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • Mutation
    S159A, S161A, T163A
  • GenBank ID
    NM_021960
  • Entrez Gene
    MCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BAMH1 (not destroyed)
  • 3′ cloning site ECOR1 (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original plasmid is from Roger Davies lab, obtained from Addgene. The mutations were done in Dr Khan's lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag- MCL1-3A (S159, S162, T163 mutated to A) was a gift from Omar Khan (Addgene plasmid # 197458 ; http://n2t.net/addgene:197458 ; RRID:Addgene_197458)

  • For your References section:

    E3 Ubiquitin Ligase TRIP12 Controls Exit from Mitosis via Positive Regulation of MCL-1 in Response to Taxol. Keyan KS, Alanany R, Kohil A, Khan OM. Cancers (Basel). 2023 Jan 13;15(2):505. doi: 10.3390/cancers15020505. 10.3390/cancers15020505 PubMed 36672454
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