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Addgene

sgCRY2
(Plasmid #197457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-hU6-gRNA-hSyn-mCherry-KASH
  • Backbone size w/o insert (bp) 5294
  • Total vector size (bp) 5699
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hU6-sgCRY2-1-hU6-sgCRY2-2
  • Alt name
    sgCRY2
  • gRNA/shRNA sequence
    CTGTGGGCATCAACCGATGG, TCGGTTGATGCCCACAGACG
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cry2 (a.k.a. D130054K12Rik)
  • Promoter U6, hSyn
  • Tag / Fusion Protein
    • mCherry (K14E, N210D, E249G) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu1 (not destroyed)
  • 3′ cloning site Age1 (not destroyed)
  • 5′ sequencing primer ITR_R CGGCCTCAGTGAGCGA
  • 3′ sequencing primer hSyn_R gtcggtcgtcaggtaggcac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the mutations K14E, N210D, and E249G were found in the mCherry sequence. The fluorophore is still fluorescent, as described in the publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sgCRY2 was a gift from Han Kyoung Choe (Addgene plasmid # 197457 ; http://n2t.net/addgene:197457 ; RRID:Addgene_197457)

  • For your References section:

    Multiplexed CRISPR-Cas9 system in a single adeno-associated virus to simultaneously knock out redundant clock genes. Kim B, Kim J, Chun M, Park I, Kwak D, Choi M, Kim K, Choe HK. Sci Rep. 2021 Jan 28;11(1):2575. doi: 10.1038/s41598-021-82287-0. 10.1038/s41598-021-82287-0 PubMed 33510438
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