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pDEST-GST-FBW7 alpha
(Plasmid #197456)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197456 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST-565
  • Vector type
    Bacterial Expression ; In vitro translation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Used mainly for in vitro translation.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FBW7
  • Alt name
    hCDC4, FBW7 isoform 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Mutation
    See depositor comments below
  • GenBank ID
    NM_001349798.2
  • Entrez Gene
    FBXW7 (a.k.a. AGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)
  • Promoter T7
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note, plasmid contains an A871T mutation in FBW7. This mutation is outside of the WD40 propeller and is not expected to interfere with the function of the protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-GST-FBW7 alpha was a gift from Omar Khan (Addgene plasmid # 197456 ; http://n2t.net/addgene:197456 ; RRID:Addgene_197456)

  • For your References section:

    Proteasomal degradation of the tumour suppressor FBW7 requires branched ubiquitylation by TRIP12. Khan OM, Almagro J, Nelson JK, Horswell S, Encheva V, Keyan KS, Clurman BE, Snijders AP, Behrens A. Nat Commun. 2021 Apr 6;12(1):2043. doi: 10.1038/s41467-021-22319-5. 10.1038/s41467-021-22319-5 PubMed 33824312
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