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Addgene

pDEST-Myc-FBW7 WD40 (All deleted)
(Plasmid #197455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST-Myc
  • Vector type
    Mammalian Expression
  • Selectable markers
    not known.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FBW7
  • Alt name
    hCDC4, FBW7 isoform 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    FBW& dimerization domain deleted.
  • GenBank ID
    NM_001349798.2
  • Entrez Gene
    FBXW7 (a.k.a. AGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-Myc-FBW7 WD40 (All deleted) was a gift from Omar Khan (Addgene plasmid # 197455 ; http://n2t.net/addgene:197455 ; RRID:Addgene_197455)

  • For your References section:

    Proteasomal degradation of the tumour suppressor FBW7 requires branched ubiquitylation by TRIP12. Khan OM, Almagro J, Nelson JK, Horswell S, Encheva V, Keyan KS, Clurman BE, Snijders AP, Behrens A. Nat Commun. 2021 Apr 6;12(1):2043. doi: 10.1038/s41467-021-22319-5. 10.1038/s41467-021-22319-5 PubMed 33824312
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