MSCV FLIP FF
(Plasmid #19744)

• Depositing Lab: Richard Hynes
• Publication: Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.

Backbone

• Vector backbone: MSCV FLIP
• Backbone size w/o insert (bp): 7926
• Vector type: Mammalian Expression, Retroviral, RNAi, Cre/Lox
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: grow in stbl3 cells at 30oC
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: oligo targeting Firefly luciferase
• Insert Size (bp): 386

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: GFP miR primer (GCTGGAGTTCGTGACCGCC)

Resource Information

• Supplemental Documents:
  • FLIP vector protocols

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constitutively expresses puromycin-resistance and the surface molecule Thy1.1. Recombines to express GFP and miR30-based RNAi.

There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
This is the control vector, which can be used for cloning. This vector expresses Firefly luciferase.

See FLIP vector protocols (PDF from this page) for more information.