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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2
(Plasmid #197422)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197422 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSpCas9(BB)-2A-mCherry
  • Total vector size (bp) 9303
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAPRE3 gRNA #2 (targets Exon 1)
  • Alt name
    EB3
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001303050.1
  • Entrez Gene
    MAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2 was a gift from Torsten Wittmann (Addgene plasmid # 197422 ; http://n2t.net/addgene:197422 ; RRID:Addgene_197422)

  • For your References section:

    Growth cone advance requires EB1 as revealed by genomic replacement with a light-sensitive variant. Dema A, Charafeddine R, Rahgozar S, van Haren J, Wittmann T. Elife. 2023 Jan 30;12:e84143. doi: 10.7554/eLife.84143. 10.7554/eLife.84143 PubMed 36715499
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