hBax S184A C3-EGFP
(Plasmid #19742)

• Depositing Lab: Richard Youle
• Publication: Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Backbone

• Vector backbone: C3-EGFP
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bax S184A
• Alt name: BCL2-associated X protein
• Species: H. sapiens (human)
• Insert Size (bp): 579
• Mutation: Serine 184 to Alanine
• GenBank ID: NM_138761
• Entrez Gene: BAX (a.k.a. BCL2L4)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry