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Addgene

PX333-T2A-EGFP
(Plasmid #197417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197417 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX333
  • Backbone manufacturer
    Addgene 64073
  • Modifications to backbone
    T2A-EGFP added to SpCas9
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter CBh; U6

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Andrea Ventura (pX333, Addgene plasmid # 64073) and Feng Zhang (pSpCas9(BB)-2A-GFP (PX458), Addgene plasmid # 48138).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PX333-T2A-EGFP was a gift from Yeh-Hsing Lao (Addgene plasmid # 197417 ; http://n2t.net/addgene:197417 ; RRID:Addgene_197417)

  • For your References section:

    Phenylboronic Acid-Functionalized Polyplexes Tailored to Oral CRISPR Delivery. Yoshinaga N, Zhou JK, Xu C, Quek CH, Zhu Y, Tang D, Hung LY, Najjar SA, Shiu CYA, Margolis KG, Lao YH, Leong KW. Nano Lett. 2023 Jan 17. doi: 10.1021/acs.nanolett.2c02306. 10.1021/acs.nanolett.2c02306 PubMed 36648291
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