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pRosa26 LSL luciferase targeting
(Plasmid #19740)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19740 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    modified pRosa26.1
  • Backbone manufacturer
    pRosa26.1 from Soriano Lab
  • Vector type
    Mammalian Expression, Mouse Targeting, Cre/Lox, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow at 30oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Species
    firefly
  • Insert Size (bp)
    1700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Luciferase from Promega's pGL3-Basic vector

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pRosa26 Lox Stop Lox Luciferase targeting vector.

Backbone is the pRosa26.1 vector from the Soriano lab
This includes both 5' and 3' Rosa26 targeting arms as well as PGK-DTA negative selection marker. The SacII site of the original vector has been deleted and replaced with a linearization cassette.
This includes AscI, PacI, and FseI restriction sites.

On the sequence provided by the author:
1-1090 Rosa26 5'-homology arm;
1091-1606 CAGGS promoter (what I could sequence of it; there should be another 1-1.3kb unsequenced - NNNN); 1607-8024 mockLoxP flanked PuroR & STOP cassette (Puro transcription is antisense to CAGGS); 8077-9726 Firefly Luciferase coding sequence (no STOP codon); 9727-9777 SYRGL epitope sequence fused to Luc and followed by STOP codon; 9778-9829 synthetic PMCpolyA; 9830-14117 Rosa26 3'-homology arm

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRosa26 LSL luciferase targeting was a gift from Tyler Jacks (Addgene plasmid # 19740)
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