RUFY1-GFP
(Plasmid
#197394)
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PurposeExpresses RUFY1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRUFY1
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SpeciesH. sapiens (human)
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Entrez GeneRUFY1 (a.k.a. RABIP4, ZFYVE12)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GFP-N-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RUFY1-GFP was a gift from Juan Bonifacino (Addgene plasmid # 197394 ; http://n2t.net/addgene:197394 ; RRID:Addgene_197394) -
For your References section:
RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin. Keren-Kaplan T, Saric A, Ghosh S, Williamson CD, Jia R, Li Y, Bonifacino JS. Nat Commun. 2022 Mar 21;13(1):1506. doi: 10.1038/s41467-022-28952-y. 10.1038/s41467-022-28952-y PubMed 35314674
