SNX19-GFP
(Plasmid
#197392)
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PurposeExpresses SNX19 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197392 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneGFP
- Backbone size w/o insert (bp) 3400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSNX19
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SpeciesH. sapiens (human)
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Entrez GeneSNX19 (a.k.a. CHET8)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GFP-N-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SNX19-GFP was a gift from Juan Bonifacino (Addgene plasmid # 197392 ; http://n2t.net/addgene:197392 ; RRID:Addgene_197392) -
For your References section:
SNX19 restricts endolysosome motility through contacts with the endoplasmic reticulum. Saric A, Freeman SA, Williamson CD, Jarnik M, Guardia CM, Fernandopulle MS, Gershlick DC, Bonifacino JS. Nat Commun. 2021 Jul 27;12(1):4552. doi: 10.1038/s41467-021-24709-1. 10.1038/s41467-021-24709-1 PubMed 34315878
