pLenti-TetO-PM
(Plasmid #197387)

• Purpose: To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)
• Depositing Lab: Qiao Zhou
• Publication: Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038/s41556-023-01130-y.

Backbone

• Vector backbone: pCW57.1
• Backbone size w/o insert (bp): 7449
• Total vector size (bp): 9453
• Vector type: Mammalian Expression, Lentiviral ; TetO
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PDX1-T2A-MAFA
• Species: M. musculus (mouse)
• Insert Size (bp): 2004
• GenBank ID: NM_008814.4 NM_194350.2
• Promoter: TRE

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TRE-F
• 3′ sequencing primer: hPGK-R

Resource Information

• Supplemental Documents:
  • pLenti-TetO-PM.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry