pLenti-EF1a-Ngn3ER-PuroR-mCherry
(Plasmid
#197385)
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PurposeTo establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197385 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin ; mCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNgn3ER-2A-PuroR-2A-mCherry
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SpeciesM. musculus (mouse)
- Promoter EF1A
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EF1A-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-EF1a-Ngn3ER-PuroR-mCherry was a gift from Qiao Zhou (Addgene plasmid # 197385 ; http://n2t.net/addgene:197385 ; RRID:Addgene_197385) -
For your References section:
Stomach-derived human insulin-secreting organoids restore glucose homeostasis. Huang X, Gu W, Zhang J, Lan Y, Colarusso JL, Li S, Pertl C, Lu J, Kim H, Zhu J, Breault DT, Sevigny J, Zhou Q. Nat Cell Biol. 2023 Apr 27. doi: 10.1038/s41556-023-01130-y. 10.1038/s41556-023-01130-y PubMed 37106062
