pAAV-hPhyB621(Y276H)-P2A-m1V
(Plasmid #197377)

• Purpose: The AAV vector for PhyB621(Y276H) and m1V
• Depositing Lab: Michiyuki Matsuda
• Publication: Plasmids for KOBACHI mice project (unpublished)

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Cell Biolabs Inc
• Backbone size w/o insert (bp): 4600
• Total vector size (bp): 7255
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Phytochrome B Y276H(1-621 aa)
• Alt name: PhyB621(Y276H)
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2631
• Mutation: Human codon optimized PhyB (1-621 aa), Y276H mutation
• GenBank ID: NM_001335612.1
• Promoter: CMV
• Tag / Fusion Protein:
  • P2A-m1V (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Kazuhiro Aoki (Addgene plasmid # 100540: pCAGGS-hPhyBY276H-mCherry-HRasCT)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry