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pCSIIpuro2-H2B-iRFP
(Plasmid #197352)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197352 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSIIpuro2
  • Backbone size w/o insert (bp) 10400
  • Total vector size (bp) 11800
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    iRFP with nucleus-targeting sequence
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1360
  • Promoter EF-1α
  • Tag / Fusion Protein
    • H2B (human histone 2B) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSIIpuro2-H2B-iRFP was a gift from Michiyuki Matsuda (Addgene plasmid # 197352 ; http://n2t.net/addgene:197352 ; RRID:Addgene_197352)

  • For your References section:

    Knockout of all ErbB-family genes delineates their roles in proliferation, survival, and migration. Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, Matsuda M, Terai K. J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199. 10.1242/jcs.261199 PubMed 37519219
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