GammaPCDH-A3-GFP/pEGFP-N1
(Plasmid
#197343)
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PurposePlasmid expressing an antigen targeting Pan-Gamma-protocadherin-Constant
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepcdh-gamma-A3
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SpeciesOther
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Tag
/ Fusion Protein
- GFP (GFP observed) (C terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a validated antigen plasmid of Anti-Pan-Gamma-Protocadherin-(constant) [N159/5R].
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GammaPCDH-A3-GFP/pEGFP-N1 was a gift from Joshua Weiner (Addgene plasmid # 197343 ; http://n2t.net/addgene:197343 ; RRID:Addgene_197343) -
For your References section:
Molecular heterogeneity in the choroid plexus epithelium: the 22-member gamma-protocadherin family is differentially expressed, apically localized, and implicated in CSF regulation. Lobas MA, Helsper L, Vernon CG, Schreiner D, Zhang Y, Holtzman MJ, Thedens DR, Weiner JA. J Neurochem. 2012 Mar;120(6):913-27. doi: 10.1111/j.1471-4159.2011.07587.x. Epub 2011 Dec 7. 10.1111/j.1471-4159.2011.07587.x PubMed 22092001