pSP72-deltaFlincG
(Plasmid #19733)

• Depositing Lab: Wolfgang Dostmann
• Publication: Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):365-70.

Backbone

• Vector backbone: pSP72
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha, 37C, overnight
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: delta-FlincG
• Alt name: protein kinase, cGMP-dependent, type I
• Species: Bovine
• Insert Size (bp): 1600
• Mutation: aa 77-356
• Entrez Gene: PRKG1 (a.k.a. CGKI, cGK 1, cGKI-beta)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry