NavB4-GFP/pEGFP-N1
(Plasmid
#197310)
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PurposePlasmid expressing an antigen targeting the Nav beta4 sodium channel subunit
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197310 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNavB4-GFP
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SpeciesR. norvegicus (rat)
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Entrez GeneScn4b
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A V228L mutation was found in NavB4.
This is a validated antigen plasmid of Anti-Navbeta4 Na+ channel [N168/6.3R].
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NavB4-GFP/pEGFP-N1 was a gift from Lori Isom (Addgene plasmid # 197310 ; http://n2t.net/addgene:197310 ; RRID:Addgene_197310) -
For your References section:
Regulation of persistent Na current by interactions between beta subunits of voltage-gated Na channels. Aman TK, Grieco-Calub TM, Chen C, Rusconi R, Slat EA, Isom LL, Raman IM. J Neurosci. 2009 Feb 18;29(7):2027-42. doi: 10.1523/JNEUROSCI.4531-08.2009. 10.1523/JNEUROSCI.4531-08.2009 PubMed 19228957