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Addgene

NavB4-GFP/pEGFP-N1
(Plasmid #197310)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197310 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NavB4-GFP
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Scn4b
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A V228L mutation was found in NavB4.

This is a validated antigen plasmid of Anti-Navbeta4 Na+ channel [N168/6.3R].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NavB4-GFP/pEGFP-N1 was a gift from Lori Isom (Addgene plasmid # 197310 ; http://n2t.net/addgene:197310 ; RRID:Addgene_197310)
  • For your References section:

    Regulation of persistent Na current by interactions between beta subunits of voltage-gated Na channels. Aman TK, Grieco-Calub TM, Chen C, Rusconi R, Slat EA, Isom LL, Raman IM. J Neurosci. 2009 Feb 18;29(7):2027-42. doi: 10.1523/JNEUROSCI.4531-08.2009. 10.1523/JNEUROSCI.4531-08.2009 PubMed 19228957

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