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Addgene

pCDNA3 Flag Jnk1a1MKK7B2
(Plasmid #19731)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19731 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Jnk1a1MKK7b2
  • Alt name
    Jnk1a1
  • Alt name
    MKK7
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    2674
  • Entrez Gene
    Map2k7 (a.k.a. 5930412N11Rik, JNKK 2, Jnkk, Jnkk2, MAPKK 7, MEK 7, Mapkk7, Mek7, Mkk7, Prkm, Prkmk7, sek, sek2)
  • Entrez Gene
    MAPK8 (a.k.a. JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 Flag Jnk1a1MKK7B2 was a gift from Roger Davis (Addgene plasmid # 19731 ; http://n2t.net/addgene:19731 ; RRID:Addgene_19731)

  • For your References section:

    The Bax subfamily of Bcl2-related proteins is essential for apoptotic signal transduction by c-Jun NH(2)-terminal kinase. Lei K, Nimnual A, Zong WX, Kennedy NJ, Flavell RA, Thompson CB, Bar-Sagi D, Davis RJ. Mol Cell Biol. 2002 Jul . 22(13):4929-42. 10.1128/MCB.22.13.4929-4942.2002 PubMed 12052897
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