pSLICER
(Plasmid
#197288)
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PurposeConjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transfer
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197288 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEINO1
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Backbone manufacturerBrumwell et al.
- Backbone size w/o insert (bp) 14927
- Total vector size (bp) 16176
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Vector typeSynthetic Biology ; Bacterial and Yeast Cloning
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli Epi300
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Growth instructionsPlasmid can be induced to high copy with arabinose.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameI-SceI Endonuclease
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Insert Size (bp)1249
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.09.21.508766v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLICER was a gift from Bogumil Karas (Addgene plasmid # 197288 ; http://n2t.net/addgene:197288 ; RRID:Addgene_197288) -
For your References section:
SLICER: A Seamless Gene Deletion Method for Deinococcus radiodurans. Brumwell SL, Van Belois KD, Nucifora DP, Karas BJ. Biodes Res. 2023 Mar 15;5:0009. doi: 10.34133/bdr.0009. eCollection 2023. 10.34133/bdr.0009 PubMed 37849465
Map uploaded by the depositor.
