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pAL1
(Plasmid #197285)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197285 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 8909
  • Total vector size (bp) 10782
  • Modifications to backbone
    Addition of CEN-ARS-HIS elements for selection and replication in S. cerevisiae
  • Vector type
    Synthetic Biology ; Bacterial and Yeast Cloning
  • Selectable markers
    HIS3 ; Amplicilin

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli Epi300
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
  • Alt name
    OriC
  • Species
    Acholeplasma laidlawii
  • Insert Size (bp)
    1873

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAL1 was a gift from Bogumil Karas (Addgene plasmid # 197285 ; http://n2t.net/addgene:197285 ; RRID:Addgene_197285)

  • For your References section:

    An Expanded Genetic Toolbox to Accelerate the Creation of Acholeplasma laidlawii Driven by Synthetic Genomes. Nucifora DP, Mehta ND, Giguere DJ, Karas BJ. ACS Synth Biol. 2024 Jan 19;13(1):45-53. doi: 10.1021/acssynbio.3c00399. Epub 2023 Dec 19. 10.1021/acssynbio.3c00399 PubMed 38113213
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