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Addgene

pFsynW SYP-GFP
(Plasmid #197283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW (#14883)
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SYP and GFP
  • Species
    R. norvegicus (rat)
  • Mutation
    N/A
  • Entrez Gene
    Syp (a.k.a. Syp1)
  • Promoter hSyn (human synapsin I promoter)
  • Tag / Fusion Protein
    • 1D4 epitope (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFsynW SYP-GFP was a gift from Edwin Chapman (Addgene plasmid # 197283 ; http://n2t.net/addgene:197283 ; RRID:Addgene_197283)

  • For your References section:

    Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons. Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER. Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568. 10.7554/eLife.82568 PubMed 36729040
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