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Addgene

pEF HaloTag-SYB2
(Plasmid #197282)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197282 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF (#11154)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SYB2 and HaloTag
  • Alt name
    VAMP2
  • Species
    R. norvegicus (rat)
  • Mutation
    Q76V and F77W
  • Entrez Gene
    Vamp2 (a.k.a. RATVAMPB, RATVAMPIR, SYB, Syb2)
  • Promoter EF1alpha

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF HaloTag-SYB2 was a gift from Edwin Chapman (Addgene plasmid # 197282 ; http://n2t.net/addgene:197282 ; RRID:Addgene_197282)

  • For your References section:

    Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons. Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER. Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568. 10.7554/eLife.82568 PubMed 36729040
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