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pFsynW SYT1-PGM reporter
(Plasmid #197278)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197278 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW (#14883)
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SYT1 and HaloTag
  • Species
    R. norvegicus (rat)
  • Mutation
    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A
  • Entrez Gene
    Syt1 (a.k.a. P65)
  • Promoter hSyn (human synapsin I promoter)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFsynW SYT1-PGM reporter was a gift from Edwin Chapman (Addgene plasmid # 197278 ; http://n2t.net/addgene:197278 ; RRID:Addgene_197278)

  • For your References section:

    Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons. Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER. Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568. 10.7554/eLife.82568 PubMed 36729040
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