pFsynW SYT1 reporter
(Plasmid
#197275)
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PurposeEncodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197275 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneFUGW (#14883)
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSYT1 and HaloTag
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SpeciesR. norvegicus (rat)
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MutationN/A
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Entrez GeneSyt1 (a.k.a. P65)
- Promoter hSyn (human synapsin I promoter)
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.02.08.479521v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFsynW SYT1 reporter was a gift from Edwin Chapman (Addgene plasmid # 197275 ; http://n2t.net/addgene:197275 ; RRID:Addgene_197275) -
For your References section:
Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons. Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER. Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568. 10.7554/eLife.82568 PubMed 36729040
