pINSPECT_5'-xrRNA_NLuc_3'-XAP1
(Plasmid
#197264)
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Purpose(Empty Backbone) Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197264 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBlueScript-KS II (+)
- Backbone size (bp) 9509
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Vector typeMammalian Expression, CRISPR, Luciferase, Synthetic Biology, Unspecified
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Selectable markersPuromycin ; HSV-Thymidine kinase for counter selection using Ganciclovir after removal of selection cassette.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINSPECT_5'-xrRNA_NLuc_3'-XAP1 was a gift from Gil Westmeyer (Addgene plasmid # 197264 ; http://n2t.net/addgene:197264 ; RRID:Addgene_197264) -
For your References section:
Intron-encoded cistronic transcripts for minimally invasive monitoring of coding and non-coding RNAs. Truong DJ, Armbrust N, Geilenkeuser J, Lederer EM, Santl TH, Beyer M, Ittermann S, Steinmassl E, Dyka M, Raffl G, Phlairaharn T, Greisle T, Zivanic M, Grosch M, Drukker M, Westmeyer GG. Nat Cell Biol. 2022 Nov;24(11):1666-1676. doi: 10.1038/s41556-022-00998-6. Epub 2022 Nov 7. 10.1038/s41556-022-00998-6 PubMed 36344775
