SSB-wt
(Plasmid #197260)

• Purpose: wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance
• Depositing Lab: Timothy Lohman
• Publication: Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19. doi: 10.1016/j.jmb.2013.08.021. Epub 2013 Sep 7.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: EMB/Novagen
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 6182
• Modifications to backbone: E. coli SSB operon between T7 promoter and SSB gene
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: wt E. coli SSB
• Species: E. coli
• Insert Size (bp): 533
• GenBank ID: NP_418483.1
• Promoter: T7
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminator

Resource Information

• Supplemental Documents:
  • Autoinduction Expression Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry