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Addgene

SSB-wt
(Plasmid #197260)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197260 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    EMB/Novagen
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6182
  • Modifications to backbone
    E. coli SSB operon between T7 promoter and SSB gene
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    wt E. coli SSB
  • Species
    E. coli
  • Insert Size (bp)
    533
  • GenBank ID
    NP_418483.1
  • Promoter T7
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SSB-wt was a gift from Timothy Lohman (Addgene plasmid # 197260 ; http://n2t.net/addgene:197260 ; RRID:Addgene_197260)
  • For your References section:

    Multiple C-terminal tails within a single E. coli SSB homotetramer coordinate DNA replication and repair. Antony E, Weiland E, Yuan Q, Manhart CM, Nguyen B, Kozlov AG, McHenry CS, Lohman TM. J Mol Biol. 2013 Nov 29;425(23):4802-19. doi: 10.1016/j.jmb.2013.08.021. Epub 2013 Sep 7. 10.1016/j.jmb.2013.08.021 PubMed 24021816