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Addgene

pGADT7-δ
(Plasmid #197259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGADT7
  • Backbone size w/o insert (bp) 7987
  • Total vector size (bp) 11451
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AP-3 δ
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3462
  • Mutation
    Val 549 Ile substitution; silent substitutions in codons 663, 867 and 876
  • Entrez Gene
    AP3D1 (a.k.a. ADTD, HPS10, hBLVR)
  • Promoter ADH1
  • Tags / Fusion Proteins
    • GAL4 transcriptional activation domain (AD) fragment (N terminal on backbone)
    • HA tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GAL4 transcriptional activation domain forward
  • 3′ sequencing primer GAL4 transcriptional activation domain reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGADT7-δ was a gift from Juan Bonifacino (Addgene plasmid # 197259 ; http://n2t.net/addgene:197259 ; RRID:Addgene_197259)

  • For your References section:

    Conservation and diversification of dileucine signal recognition by adaptor protein (AP) complex variants. Mattera R, Boehm M, Chaudhuri R, Prabhu Y, Bonifacino JS. J Biol Chem. 2011 Jan 21;286(3):2022-30. doi: 10.1074/jbc.M110.197178. Epub 2010 Nov 19. 10.1074/jbc.M110.197178 PubMed 21097499
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