pmiRFP680-Actin-C-18
(Plasmid
#197219)
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PurposeExpresses the fusion protein of miRFP680-Actin-C-18 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197219 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepN1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemiRFP680-Actin-C-18
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.researchsquare.com/article/rs-797586/v1 for preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmiRFP680-Actin-C-18 was a gift from Kiryl Piatkevich (Addgene plasmid # 197219 ; http://n2t.net/addgene:197219 ; RRID:Addgene_197219) -
For your References section:
Quantitative assessment of near-infrared fluorescent proteins. Zhang H, Papadaki S, Sun X, Wang X, Drobizhev M, Yao L, Rehbock M, Koster RW, Wu L, Namikawa K, Piatkevich KD. Nat Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z. 10.1038/s41592-023-01975-z PubMed 37666982