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Addgene

pBabe puro RalA Q75L
(Plasmid #19719)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19719 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBabe
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    V-ral simian leukemia viral oncogene homolog A
  • Alt name
    RalA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    630
  • Mutation
    GTPase deficient & constitutively active Glutamine to Leucine at a.a. position 75. NOTE: long splice variant with 3bp deletion and 12bp insertion at 5' of gene. Long Sp. Variant: MVDYLAN Short Sp. Variant: MAAN
  • GenBank ID
    NM_005402
  • Entrez Gene
    RALA (a.k.a. HINCONS, RAL)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pBabe 5'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe puro RalA Q75L was a gift from Channing Der (Addgene plasmid # 19719 ; http://n2t.net/addgene:19719 ; RRID:Addgene_19719)
  • For your References section:

    Divergent roles for RalA and RalB in malignant growth of human pancreatic carcinoma cells. Lim KH, O'Hayer K, Adam SJ, Kendall SD, Campbell PM, Der CJ, Counter CM. Curr Biol. 2006 Dec 19. 16(24):2385-94. 10.1016/j.cub.2006.10.023 PubMed 17174914