pBabe puro RalA Q75L
(Plasmid #19719)

• Depositing Lab: Channing Der
• Publication: Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Backbone

• Vector backbone: pBabe
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: V-ral simian leukemia viral oncogene homolog A
• Alt name: RalA
• Species: H. sapiens (human)
• Insert Size (bp): 630
• Mutation: GTPase deficient & constitutively active Glutamine to Leucine at a.a. position 75. NOTE: long splice variant with 3bp deletion and 12bp insertion at 5' of gene. Long Sp. Variant: MVDYLAN Short Sp. Variant: MAAN
• GenBank ID: NM_005402
• Entrez Gene: RALA (a.k.a. HINCONS, RAL)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: pBabe 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry