pEGFP-C1 Nodamura B2
(Plasmid #19716)

• Depositing Lab: Christopher Sullivan
• Publication: Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nodamura B2
• Species: Nodamura Virus
• Insert Size (bp): 429
• Mutation: 2 silent point mutations
• Entrez Gene: NoVsRNA1gp2
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • Plasmid map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Nodamuravirus B2 gene is subcloned from the KpnI/ApaI fragment from pcDNA3.1+puro Nodamura B2 into pEGFP-C1 vector, making the EGFP fusion construct.