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pRK5 Myc-tagged PSK (K57A)
(Plasmid #197150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    Alan Hall
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 8405
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAOK2
  • Alt name
    PSK1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3705
  • Mutation
    Changed K 57 to A
  • GenBank ID
    9344
  • Entrez Gene
    TAOK2 (a.k.a. MAP3K17, PSK, PSK1, PSK1-BETA, TAO1, TAO2, Tao2beta)
  • Promoter SV40
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer GGTCCAACTGCACCTCGG
  • 3′ sequencing primer GCAATAAACAAGTTGGGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned by Jonathan Morris at KCL (subject to MTA with Addgene).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plese note: This construct originated from a T47-D breast carcinoma cell cDNA library and contains the R1211H variant in PSK1/TAOK2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 Myc-tagged PSK (K57A) was a gift from Jonathan Morris (Addgene plasmid # 197150 ; http://n2t.net/addgene:197150 ; RRID:Addgene_197150)

  • For your References section:

    PSK, a novel STE20-like kinase derived from prostatic carcinoma that activates the c-Jun N-terminal kinase mitogen-activated protein kinase pathway and regulates actin cytoskeletal organization. Moore TM, Garg R, Johnson C, Coptcoat MJ, Ridley AJ, Morris JD. J Biol Chem. 2000 Feb 11;275(6):4311-22. doi: 10.1074/jbc.275.6.4311. 10.1074/jbc.275.6.4311 PubMed 10660600
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