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Addgene

pNM1325
(Plasmid #197144)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197144 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPBbsr_MCS
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9-PmCDA1-UGI
  • Species
    Synthetic
  • Mutation
    SpCas9(D10A)
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

https://benchling.com/s/seq-vndGxR67s2R3wsFg99oD?m=slm-R0zhyyxggV5jJqBJjzo9
Please visit https://www.biorxiv.org/content/10.1101/2023.01.18.524633v1 for bioRxiv preprint. This plasmid is relatively unstable due to repetitive sequences and other genetic element cloned from retrovirus. Please be sure to use NEB stable cells and incubate at 30C.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNM1325 was a gift from Nozomu Yachie (Addgene plasmid # 197144 ; http://n2t.net/addgene:197144 ; RRID:Addgene_197144)

  • For your References section:

    A multi-kingdom genetic barcoding system for precise target clone isolation. Ishiguro S, Ishida K, Sakata RC, Mori H, Takana M, King S, Bashth O, Ichiraku M, Masuyama N, Takimoto R, Kijima Y, Adel A, Toyoshima H, Seki M, Oh JH, Archambault AS, Nishida K, Kondo A, Kuhara S, Aburatani H, Klein Geltink RI, Takashima Y, Shakiba N, Yachie N. bioRxiv 2023.01.18.524633 10.1101/2023.01.18.524633
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