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Addgene

CAG-Brn2-DBD-EnR
(Plasmid #19714)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19714 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CAGEN
  • Backbone size w/o insert (bp) 4798
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fly engrailed 36240
  • Alt name
    Mouse Pou3f2 18993
  • Species
    M. musculus (mouse), D. melanogaster (fly)
  • Insert Size (bp)
    1406
  • Mutation
    mouse Brn2 DNA binding domain-Drosophila engrailed repressor domain
  • Entrez Gene
    Pou3f2 (a.k.a. 9430075J19Rik, A230098E07Rik, Brn-2, Brn2, OTF-7, Otf7, oct-7)
  • Entrez Gene
    en (a.k.a. Dmel_CG9015, 153867_at, Apa, CG9015, Dmel\CG9015, EN, En, EnR, Eng, Engr, Engrailed, Engrailed/Invected, Es, V, en1, spa2, spt)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CAG-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG-Brn2-DBD-EnR was a gift from Connie Cepko (Addgene plasmid # 19714 ; http://n2t.net/addgene:19714 ; RRID:Addgene_19714)

  • For your References section:

    A core paired-type and POU homeodomain-containing transcription factor program drives retinal bipolar cell gene expression. Kim DS, Matsuda T, Cepko CL. J Neurosci. 2008 Jul 30. 28(31):7748-64. 10.1523/JNEUROSCI.0397-08.2008 PubMed 18667607
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