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Addgene

pSI-218
(Plasmid #197139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • SpCas9-PmCDA1-UGI (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    SpCas9(D10A)
  • Tag / Fusion Protein
    • SpCas9-PmCDA1-UGI (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

https://benchling.com/s/seq-ir6EdyAiwwADqkZoUTvT?m=slm-OO1VK36RD5vy05CbCzFP
Please visit https://www.biorxiv.org/content/10.1101/2023.01.18.524633v1 for bioRxiv preprint. This plasmid is relatively unstable due to repetitive sequences and other genetic element cloned from retrovirus. Please be sure to use NEB stable cells and incubate at 30C.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSI-218 was a gift from Nozomu Yachie (Addgene plasmid # 197139 ; http://n2t.net/addgene:197139 ; RRID:Addgene_197139)

  • For your References section:

    A multi-kingdom genetic barcoding system for precise target clone isolation. Ishiguro S, Ishida K, Sakata RC, Mori H, Takana M, King S, Bashth O, Ichiraku M, Masuyama N, Takimoto R, Kijima Y, Adel A, Toyoshima H, Seki M, Oh JH, Archambault AS, Nishida K, Kondo A, Kuhara S, Aburatani H, Klein Geltink RI, Takashima Y, Shakiba N, Yachie N. bioRxiv 2023.01.18.524633 10.1101/2023.01.18.524633
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