pRK5-Myc-PSK2 (1-416) (K57A)
(Plasmid #197124)

• Purpose: Expression of kinase-deficient Myc-tagged PSK2 (aa1-416, K57A) / TAOK1 (aa1-416, K57A) in mammalian cells
• Depositing Lab: Jonathan Morris
• Publication: Tavares et al J Biol Chem. 2013 May 24;288(21):15418-29. doi: 10.1074/jbc.M112.448183. Epub 2013 Apr 12.

Backbone

• Vector backbone: pRK5
• Backbone manufacturer: Alan Hall
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5948
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TAOK1 (aa1-416, K57A)
• Alt name: PSK2 (aa1-416, K57A)
• Alt name: KIAA1361 (aa1-416, K57A)
• Species: H. sapiens (human)
• Insert Size (bp): 1248
• Mutation: Changed K 57 to A and deleted C-terminus
• GenBank ID: 57551
• Entrez Gene: TAOK1 (a.k.a. DDIB, KFC-B, MAP3K16, MARKK, PSK-2, PSK2, TAO1, hKFC-B, hTAOK1)
• Promoter: SV40
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: GGTCCAACTGCACCTCGG
• 3′ sequencing primer: GCAATAAACAAGTTGGGC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned by Jonathan Morris at KCL (subject to MTA with Addgene).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry